Capillary Electrophoresis
electrophoresis (CZE), Capillary electrophoresis (CE) is a group of related separation techniques that use narrow-bone fused-silica capillaries to separate a complex array of large and small molecules. Capillary electrophoresis (CE) is used for the analysis of ions, and both basic and chiral pharmaceuticals. There are many types of capillary electrophoresis (CE) depending on the type of capillaries and the electrolytes used. Examples include micellular electrokinetic chromatography (MEKC), capillary zonecapillary gel electrophoresis (CGE), and capillary isoelectric focusing (CIEF). Micellular electrokinetic chromatography (MEKC) is used for separating mixtures containing both ionic and neutral species. Capillary zone electrophoresis (CZE) is known as free solution CE and it provides constant field strength throughout the length of the capillary. A capillary gel electrophoresis (CGE) is used to separate the proteins and nucleic acids. Capillary isoelectric focusing (CIEF) separates amphoteric molecules such as proteins by electrophoresis in a pH gradient that is generated between cathode and the anode. Other capillary electrophoresis (CE) are commonly available.
There are several ways in which capillary electrophoresis (CE) function. In micellular electrokinetic chromatography (MEKC), the surfactants are added to a buffer solution and the separation is done by differential partition between the solvent and micelle. Capillary zone electrophoresis (CZE) fills the capillary tube with aqueous buffer solution that contains both positive and negative ions. The surface of the capillary has a net negative charge because of Si-O chemical groups present in the glasslike material. Capillary gel electrophoresis (CGE) uses gel which builds heat and is detrimental to separation. It is used for DNA sequencing and genotyping. Capillary isoelectric focusing (CIEF) combines with capillary reversed-phase liquid chromatography (CRPLC) using a micro injector as the interface for performing the 2-D protein separations from complex protein mixtures. Capillary electrophoresis (CE) is designed and manufactured to meet most industry specifications.
Capillary electrophoresis (CE) is used in many applications. Some examples include genetic analysis, analysis of pharmaceuticals (containing nitrogenous bases), gunshot residue analysis, and counter-ion analysis in drug discovery. Capillary electrophoresis (CE) adheres to ICC standards.
